【皮下填充物文献】透明质酸填充剂的人体动力学以及生物刺激作用研究
Evaluation of the In Vivo Kinetics
【 文献重点摘要 】
Background: Because subcutaneously injected hyaluronic acid filler is absorbed over 6 months to 1 year after the treatment of facial wrinkles, frequent retreatment may be required. However, persistent long-term effects are often clinically observed when hyaluronic acid filler is injected as a bolus for facial augmentation. Therefore, the authors investigated, over time, the changes in volume and histologic features of subcutaneous bolus injections of hyaluronic acid.
Methods: Hyaluronic acid filler was subcutaneously injected as a bolus into the dorsum of 6-week-old rats. At several time points (immediately after injection and 4, 8, 16, 32, and 64 weeks thereafter), magnetic resonance imaging was introduced to observe morphologic changes and to measure volume. Histologic examination of sectioned tissues was also performed.
Results: The average volume increased for up to 4 weeks after injection and then gradually decreased, with 74.8 percent of the injected volume remaining after 64 weeks, with no statistical difference compared to the initial volume. Histologic analysis revealed that lattice structures were created by fibroblasts and collagen fibers, and blood vessels and adipocytes were also generated in the filler.
Conclusions: Although subcutaneous bolus injections of hyaluronic acid filler exhibited flattening, the total volume was maintained even after 64 weeks. Histologically, hyaluronic acid filler acted as a scaffold for autogenous tissue replacement by means of fibroblast migration and proliferation, collagen induction, and angiogenesis, followed by proliferation of adipocytes. This study demonstrates that the total volume is maintained long-term by replacing part of the injected hyaluronic acid filler with autologous tissues.
背景:由于皮下注射透明质酸填充剂在面部皱纹治疗后6个月至1年内被吸收,可能需要频繁的再次治疗。然而,临床上经常观察到当注射透明质酸填充剂作为面部隆起的丸剂时,持续的长期效果。因此,作者调查了随着时间的推移,皮下注射透明质酸的体积和组织学特征的变化。
方法:6周龄大鼠背部皮下注射透明质酸填充剂。在几个时间点(注射后即刻以及之后的4、8、16、32和64周),引入磁共振成像来观察形态学变化和测量体积。同时进行切片组织学检查。
结果:平均注射量在注射后4周内增加,然后逐渐减少,64周后仍有74.8%的注射量,与注射前相比无统计学差异。组织学分析显示,纤维母细胞和胶原纤维形成了晶格结构,填充物中还产生了血管和脂肪细胞。
结论:虽然皮下注射透明质酸填充剂表现出扁平,但总容量即使在64周后仍保持不变。组织学上,透明质酸填充物通过成纤维细胞的迁移增殖、胶原诱导和血管生成,作为自体组织替代的支架,继而促进脂肪细胞的增殖。这项研究表明,用自体组织替换部分注射的透明质酸填充物可以长期保持总容量。